Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of CACNA2D3 Antibody in HepG2 cell line lysates (35ug/lane)

CACNA2D3 Antibody

CATALOG NUMBER: 64-087

Clonality:

Polyclonal

Tested Applications:

Flow, WB

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Homology:

Predicted species reactivity based on immunogen sequence: Mouse, Rat

Immunogen:

This CACNA2D3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1026-1053 amino acids from the C-terminal region of human CACNA2D3.

Conjugate:

Unconjugated

Tested Applications:

Flow, WB

Application Note:

For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50

Predicted Molecular Weight:

123 kDa

Advanced Validation

Properties

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

CACNA2D3

Additional Names:

Voltage-dependent calcium channel subunit alpha-2/delta-3, Voltage-gated calcium channel subunit alpha-2/delta-3, Voltage-dependent calcium channel subunit alpha-2-3, Voltage-dependent calcium channel subunit delta-3, CACNA2D3

Protein Accession Number:

Q8IZS8

Protein Gi Number:

74723683

Ncbi Gene Id Number:

55799

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

CACNA2D3 is a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits.

Background Reference 1:

Trynka, G., et al. Gut 58(8):1078-1083(2009)

Background Reference 2:

Wanajo, A., et al. Gastroenterology 135(2):580-590(2008)

Background Reference 3:

Uhl, G.R., et al. Arch. Gen. Psychiatry 65(6):683-693(2008)

Background Reference 4:

Abo-Dalo, B., et al. Am. J. Med. Genet.143A (22), 2668-2674 (2007)

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-087

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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CACNA2D3 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Homology:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

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