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45-351 (0.2ug/ml) staining of human bone marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing CACNB4 and probed with 45-351 (mock transfection in first lane).
45-351 (0.2ug/ml) staining of human bone marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CACNB4 Antibody

CATALOG NUMBER: 45-351

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig
Immunogen:
The immunogen for this antibody is: CSPGGYSHDSRHRL
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 60+55kDa bands observed in Human Bone Marrow lysates and in transfected HEK293 transiently expressing CACNB4 (calculated MW of 58.2kDa according to NP_000717.2 and 54.7kDa according to NP_001005747.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognise all reported protein isoforms of human CACNB4 (NP_001005747.1; NP_000717.2; NP_001005746.1; )
Positive Control 1:
Human Bone Marrow Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CACNB4
Additional Names:
CACNB4, CAB4, CACNLB4, calcium channel, voltage-dependent, beta 4 subunit, dihydropyridine-sensitive L-type, calcium channel beta-4 subunit, EA5, EIG9, EJM, EJM4, EJM6, OTTHUMP00000207247, OTTHUMP00000207249
Protein Accession Number:
NP_001005747.1,NP_000717.2,NP_001005746.1
Protein Gi Number:
54607068
Ncbi Gene Id Number:
785
Background Reference 1:
Badou A, Basavappa S, Desai R, Peng YQ, Matza D, Mehal WZ, Kaczmarek LK, Boulpaep EL, Flavell RA. Requirement of voltage-gated calcium channel beta4 subunit for T lymphocyte functions. Science. 2005 Jan 7;307(5706):117-21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-351

List Size:
0.1 mg

List Price:

$430.00

Out of stock

Datasheet
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