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46-822 (1ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-822 (1ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Cadherin-like 23 Antibody

CATALOG NUMBER: 46-822

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-YNISLYENVTVGTS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 60kDa band observed in Human Brain (Amygdala and Frontal Cortex lysates (calculated MW of 58.6kDa according to NP_443068.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_071407.3; NP_443068.1).
Positive Control 1:
Cat. No. XBL-10131 - Human Amygdala Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CDH23
Additional Names:
CDH23, USH1D, cadherin-like 23, DFNB12, DKFZp434P2350, FLJ00233, FLJ36499, KIAA1774, KIAA1812, MGC102761, USH1H, OTTHUMP00000044780, cadherin 23, cadherin related 23, cadherin-23, otocadherin
Protein Accession Number:
NP_071407.3,NP_443068.1
Protein Gi Number:
153251203
Ncbi Gene Id Number:
64072
Background Reference 1:
Roux AF, Faugère V, Le Guedard S, Pallares-Ruiz N, Vielle A, Chambert S, Marlin S, Hamel C, Gilbert B, Malcolm S, Claustres M; French Usher SyndromeCollaboration. Survey of the frequency of USH1 gene mutations in a cohort of Usher patients shows the importance of cadherin 23 and protocadherin 15 genes and establishes a detection rate of above 90%. J Med Genet. 2006 Sep;43(9):763-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-822

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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