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43-334 (1ug/ml) staining of Human Colorectal cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-334 (1ug/ml) staining of Human Colorectal cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CADM1 Antibody

CATALOG NUMBER: 43-334

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NLQTQRYLEVQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60-65kDa double band observed in Human Colorectal cancer lysates (calculated MW of 48.5kDa according to NP_055148.3). The observed sizes correspond to glycosylated forms. Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_055148.3; NP_001091987.1).
Predicted Molecular Weight:
Approx 60-65kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CADM1
Additional Names:
CADM1, cell adhesion molecule 1, BL2, DKFZp686F1789, IGSF4, IGSF4A, MGC149785, MGC51880, NECL2, Necl-2, RA175, ST17, SYNCAM, TSLC1, sTSLC-1, sgIGSF, synCAM1, TSLC1/Nectin-like 2/IGSF4, immunoglobulin superfamily, member 4, nectin-like 2, nectin-like protein 2, tumor suppressor in lung cancer 1
Protein Accession Number:
NP_055148.3,NP_001091987.1
Protein Gi Number:
148664190
Ncbi Gene Id Number:
23705
Background Reference 1:
Berginc G, Bracko M, Glavac D, MS-MLPA reveals progressive age-dependent promoter methylation of tumor suppressor genes and possible role of IGSF4 gene in colorectal carcinogenesis of microsatellite instable tumors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-334

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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