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Staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). A) EB08040 (0.1ug/ml) and B) 45-353 (0.05ug/ml). Detected by chemiluminescence.
45-353 (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-353 (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). A) EB08040 (0.1ug/ml) and B) 45-353 (0.05ug/ml). Detected by chemiluminescence.

CADM4 Antibody

CATALOG NUMBER: 45-353

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-REAFLNGSDGHKRKE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 42.8kDa according to NP_660339.1). The observed molecular weight corresponds to findings with EB08040, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Liver. Recommended concentration: 2.5ug/ml.
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CADM4
Additional Names:
CADM4, cell adhesion molecule 4 , IGSF4C, Necl-4, TSLL2, synCAM4, TSLC1-like 2, immunoglobulin superfamily, member 4C, nectin-like 4
Protein Accession Number:
NP_660339.1
Protein Gi Number:
21686977
Ncbi Gene Id Number:
199731
Background Reference 1:
Spiegel I, Adamsky K, Eshed Y, Milo R, Sabanay H, Sarig-Nadir O, Horresh I, Scherer SS, Rasband MN, Peles E. A central role for Necl4 (SynCAM4) in Schwann cell-axon interaction and myelination. Nat Neurosci. 2007 Jul;10(7):861-9. Epub 2007 Jun 3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-353

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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