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Western blot analysis of extracts of various cell lines, using CALB2 antibody (23-912) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded mouse kidney using CALB2 antibody (23-912) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CALB2 antibody (23-912) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat brain using CALB2 antibody (23-912) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using CALB2 antibody (23-912) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CALB2 antibody (23-912) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

CALB2 Antibody

CATALOG NUMBER: 23-912

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human CALB2 (NP_001731.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:1000 - 1:4000
IHC: 1:50 - 1:100
IF: 1:50 - 1:200
Positive Control 1:
SW480
Positive Control 2:
Mouse eye
Positive Control 3:
Mouse brain
Positive Control 4:
Rat spinal cord
Positive Control 5:
Rat brain
Positive Control 6:
Mouse small intestine
Predicted Molecular Weight:
Observed: 29kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CALB2
Additional Names:
CALB2, 29 kDa calbindin, CAL2, Calretinin, CAB29, Calbindin 2, Calbindin D29K, CR
Ncbi Gene Id Number:
794
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-912

List Size:
100 uL

List Price:

$436.00

Datasheet
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