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Western blot analysis of anti-PPP3CA Pab in mouse kidney tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-PPP3CA Pab in mouse kidney tissue lysate.

Calcineurin Antibody

CATALOG NUMBER: 63-485

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This Calcineurin (PPP3CA) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-51 amino acids from the N-terminal region of human Calcineurin (PPP3CA).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPP3CA
Additional Names:
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit alpha isoform, PPP3CA, CALNA, CNA
Protein Accession Number:
Q08209
Protein Gi Number:
1352673
Ncbi Gene Id Number:
5530
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PPP3CA is a calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. PPP3CA dephosphorylates HSPB1 and SSH1.
Background Reference 1:
Rodriguez, A., et al., J. Biol. Chem. 280(11):9980-9984 (2005). Shirane, M., et al., Nat. Cell Biol. 5(1):28-37 (2003). Bengoechea-Alonso, M.T., et al., Nitric Oxide 8(1):65-74 (2003). Huai, Q., et al., Proc. Natl. Acad. Sci. U.S.A. 99(19):12037-12042 (2002). Bennasser, Y., et al., Virology 303(1):174-180 (2002).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-485

List Size:
400 ul

List Price:

$495.00

Datasheet
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