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Western blot analysis using calcyclin monoclonal antibody against truncated calcyclin recombinant protein
Immunohistochemical analysis of paraffin - embedded human brain glioma tissue showing nuclear localization using calcyclin antibody with DAB staining.
Western blot analysis using calcyclin monoclonal antibody against truncated calcyclin recombinant protein

Calcyclin Antibody [7D11A8]

CATALOG NUMBER: 32-124

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
GSTs Resin purified truncated recombinant calcyclin expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:Dilution 1:200 - 1:1,000
IHC(P):Dilution 1:100 - 1:500
IHC(F):Dilution 1:100 - 1:500
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Calcyclin monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CACYBP
Additional Names:
S100A6BP, SIP, PNAS-107, S100A6-binding protein, CacyBP, GIG5, S100A6BP, RP1-102G20.6
Protein Accession Number:
Q9HB71
Protein Gi Number:
46576651
Ncbi Gene Id Number:
27101
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Calcyclin encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.
Background Reference 1:
Bean C.et.al J Mol Biol. 2005 Jun 3;349(2):349-66.
Background Reference 2:
HWang R.et.al J Biol Chem. 2004 May 14;279(20):21239-47.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-124

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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