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Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.

CALR Antibody

CATALOG NUMBER: 27-297

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CALR.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CALR antibody can be used for detection of CALR by ELISA at 1:312500. CALR antibody can be used for detection of CALR by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
48 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CALR antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CALR
Additional Names:
CALR, RO, CRT, SSA, cC1qR, HEL-S-99n
Protein Accession Number:
NP_004334
Protein Gi Number:
4757900
Ncbi Gene Id Number:
811
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Calreticulin is a multifunctional protein that acts as a major Ca (2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium.
Background Reference 1:
Urade, R., et al., (2004) Biochemistry 43 (27), 8858-8868.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-297

List Size:
100 ul

List Price:

$519.00

Datasheet
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