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Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~50k -subunit and ~60k β-subunit of the CaM Kinase II protein phosphorylated at Thr286.
Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~50k -subunit and ~60k β-subunit of the CaM Kinase II protein phosphorylated at Thr286.

CaM Kinase II (phospho Thr286) Antibody

CATALOG NUMBER: XPS-1000

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Frog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Frog, Human, Mouse, Rat
Immunogen:
CaM Kinase II (Thr286) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of rat brain lysates with phospho- and dephosphopeptide competition were used to establish the phospho-specificity of the antibody. Applications include Dot Blot (DB) and Western Blot (WB). Dot Blots and Western Blots performed with each lot and working dilutions internally tested under ideal conditions for DB and WB were 1:1000. Suitability for Immunohistochemistry (IHC) has not been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody.
Specificity:
CaM Kinase II antibody is specific for a 50 kDa and b 60 kDa CaM Kinase II phosphorylated at Thr286.
Predicted Molecular Weight:
50/60
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
Camk2a
Protein Accession Number:
P11275
Protein Gi Number:
125285
Ncbi Gene Id Number:
25400
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Calcium/calmodulin kinase II (CaM Kinase II) is multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers. Phosphorylation of Thr286 produces an autonomously active form of CaM Kinase II. The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.
Background Reference 1:
Kennedy, M. B., "Signal-processing machines at the postsynaptic density," Science, 290 (2000) 750-754.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1000

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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