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Antibody is used in Western blot to detect CAMK1 in HeLa cell lysate (lane 1) and primate brain tissue lysate (lane 2).
Western blot analysis of CAMK1 using rabbit polyclonal CAMK1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect CAMK1 in HeLa cell lysate (lane 1) and primate brain tissue lysate (lane 2).

CAMK1 Antibody

CATALOG NUMBER: 62-731

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
This CAMK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human CAMK1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CAMK1
Additional Names:
Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM-KI, CaM kinase I alpha, CaMKI-alpha, CAMK1
Protein Accession Number:
Q14012
Protein Gi Number:
3122301
Ncbi Gene Id Number:
8536
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
Background Reference 1:
Condon, J.C., et al., Endocrinology 143(9):3651-3657 (2002).
Background Reference 2:
Chin, D., et al., J. Biol. Chem. 272(50):31235-31240 (1997).
Background Reference 3:
Haribabu, B., et al., EMBO J. 14(15):3679-3686 (1995).
Background Reference 4:
Ishikawa, Y., et al., FEBS Lett. 550 (1-3), 57-63 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-731

List Size:
400 ul

List Price:

$495.00

Datasheet
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