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Western blot analysis of extracts of various cell lines, using CAMK1D antibody (22-996) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunofluorescence analysis of A549 cells using CAMK1D antibody (22-996).
Western blot analysis of extracts of various cell lines, using CAMK1D antibody (22-996) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.

CAMK1D Antibody

CATALOG NUMBER: 22-996

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human CAMK1D (NP_705718.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
293T
Positive Control 3:
Jurkat
Positive Control 4:
Mouse thymus
Positive Control 5:
Mouse spleen
Positive Control 6:
Rat brain
Predicted Molecular Weight:
Observed: 45kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CAMK1D
Additional Names:
CAMK1D, calcium/calmodulin-dependent protein kinase, RP11-462F15.1, CKLiK, CaM-K1, CaMKID, CaM kinase ID, CamKI-like protein kinase, OTTHUMP00000019119, OTTHUMP00000019120, OTTHUMP00000045030, CAMKID
Ncbi Gene Id Number:
57118
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the calcium/calmodulin-dependent protein kinase 1 family, a subfamily of the serine/threonine kinases. The encoded protein is a component of the calcium-regulated calmodulin-dependent protein kinase cascade. It has been associated with multiple processes including regulation of granulocyte function, activation of CREB-dependent gene transcription, aldosterone synthesis, differentiation and activation of neutrophil cells, and apoptosis of erythroleukemia cells. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-996

List Size:
100 uL

List Price:

$436.00

Datasheet
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