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42-002 (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-002 (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-002 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue
42-002 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue
42-002 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
42-002 (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

CAMK2A Antibody

CATALOG NUMBER: 42-002

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 58-60kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 55.3kDa according to Human NP_057065.2, and 54.1kDa according to to Mouse NP_803126.1 and Rat NP_037052.1). An additional band of 50kDa was observed in some Rat Brain lysates and wss successfully blocked by incubation with the immunizing peptide. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03--0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the membranes of U2OS and U251 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.

Specificity:
This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CAMK2A
Additional Names:
CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase IIA, calcium/calmodulin-dependent protein kinase type II alpha chain
Protein Accession Number:
NP_057065.2,NP_741960.1
Protein Gi Number:
25952114
Ncbi Gene Id Number:
815
Background Reference 1:
Lee CW, Lin CC, Luo SF, Lee HC, Lee IT, Aird WC, Hwang TL, Yang CM. Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and histone deacetylase 4 in human tracheal smooth muscle cells. Molecular pharmacology 2008 May 73 (5): 1454-64.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-002

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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