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Western blot analysis of anti-C in 293 cell line lysates (35ug/lane).
Western blot analysis of CAMK2A using rabbit polyclonal C using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK2A gene (Lane 2).
Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMK2A (C-term E370) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-C in 293 cell line lysates (35ug/lane).

CAMK2A Antibody

CATALOG NUMBER: 62-898

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This CAMK2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-371 amino acids from the C-terminal region of human CAMK2A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CAMK2A
Additional Names:
Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2A, CAMKA, KIAA0968
Protein Accession Number:
Q9UQM7
Protein Gi Number:
296434552
Ncbi Gene Id Number:
815
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CAMK2A belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by the gene for CAMK2A is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity.
Background Reference 1:
Lee,C.W., Mol. Pharmacol. 73 (5), 1454-1464 (2008)
Background Reference 2:
Yuan,K., Lab. Invest. 87 (9), 938-950 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-898

List Size:
400 ul

List Price:

$495.00

Datasheet
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