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Western Blot at 1:2000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
Antibody is used in Western blot to detect CAMK2 gamma in mouse heart tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western Blot at 1:2000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.

CAMK2G Antibody

CATALOG NUMBER: 62-733

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CAMK2G (CAMK2 gamma) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the C-terminal region of human CAMK2G (CAMK2 gamma).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
63 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CAMK2G
Additional Names:
Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CaMK-II subunit gamma, CAMK2G, CAMK, CAMK-II, CAMKG
Protein Accession Number:
Q13555
Protein Gi Number:
62512173
Ncbi Gene Id Number:
818
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CaM-kinase II (CAMK2) is a prominent Ser/Thr protein kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Likely autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
Background Reference 1:
Breen, M.A., et al., Biochem. Biophys. Res. Commun. 236(2):473-478 (1997). Tombes, R.M., et al., Biochim. Biophys. Acta 1355(3):281-292 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-733

List Size:
400 ul

List Price:

$495.00

Datasheet
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