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Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using CaMKII (Phospho-Thr286).
Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using CaMKII (Phospho-Thr286).

CaMKII (phospho Thr286) Antibody

CATALOG NUMBER: 79-492

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CaMKII (Phospho-Thr286) antibody was raised against a peptide sequence around phosphorylation site of threonine 286 (Q-E-T (p) -V-D) derived from Human CaMKII.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of CaMKII only when phosphorylated at threonine 286.
Predicted Molecular Weight:
50 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
Camk2a
Additional Names:
CaMKII, R74975, mKIAA0968, FNRB, MDF2, MSK12, CAMK2A, CaMKII-alpha
Protein Accession Number:
NP_001273738.1
Protein Gi Number:
558472775
Ncbi Gene Id Number:
12322
Background:
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity .
Background Reference 1:
Pak JH, et al. Proc Natl Acad Sci U S A. 2000 Oct 10; 97 (21): 11232-11237 Hudmon A, et al. J Cell Biol. Author manuscript; available in PMC 2006 May 7Miller P, et al. PLoS Biol. 2005 Apr; 3 (4): e107Runyan JD, et al. Learn Mem. 2005 Mar; 12 (2): 103-110.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-492

List Size:
0.1 mg

List Price:

$468.00

Datasheet
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