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43-259 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-259 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
43-259 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CAPN1 Antibody

CATALOG NUMBER: 43-259

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: C-NASRARSEQFINLR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in Human Kidney lysates (calculated MW of 81.9kDa according to NP_005177.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Specificity:
Reported variants represent identical protein: NP_001185797.1, NP_005177.2, NP_001185798.1.
Predicted Molecular Weight:
Approx 80kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CAPN1
Additional Names:
CAPN1, calpain 1, (mu/I) large subunit, CANP, CANP1, CANPL1, muCANP, muCL, CANP 1, calcium-activated neutral proteinase 1, calpain mu-type, calpain, large polypeptide L1, calpain-1 catalytic subunit, calpain-1 large subunit, cell proliferation-inducing gene 30 protein, cell proliferation-inducing protein 30, micromolar-calpain
Protein Accession Number:
NP_005177.2
Protein Gi Number:
115574
Ncbi Gene Id Number:
823
Background Reference 1:
Panigrahi AK, Zhang N, Mao Q, Pati D.Calpain-1 cleaves Rad21 to promote sister chromatid separation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-259

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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