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Western blot analysis of extracts from normal (control) and CAPN1 knockout (KO) HeLa cells, using CAPN1 antibody (18-193) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts from normal (control) and CAPN1 knockout (KO) HeLa cells, using CAPN1 antibody (18-193) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
KO

CAPN1 Antibody, KO Validated

CATALOG NUMBER: 18-193

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CAPN1 (NP_005177.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
HeLa
Predicted Molecular Weight:
Observed: 75kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CAPN1
Additional Names:
CAPN1, calpain 1, (mu/I) large subunit, CANP, CANP1, CANPL1, muCANP, muCL, CANP 1, calcium-activated neutral proteinase 1, calpain mu-type, calpain, large polypeptide L1, calpain-1 catalytic subunit, calpain-1 large subunit, cell proliferation-inducing ge
Ncbi Gene Id Number:
823
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-193

List Size:
100 uL

List Price:

$540.00

Out of stock

Datasheet
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