Western blot analysis of CAPN3 Antibody in HL-60 cell line lysates (35ug/lane)

CAPN3 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of CAPN3 Antibody in HL-60 cell line lysates (35ug/lane)

CAPN3 Antibody

CATALOG NUMBER: 64-130

Clonality:

Polyclonal

Tested Applications:

Flow, IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Homology:

Predicted species reactivity based on immunogen sequence: Monkey

Immunogen:

This CAPN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 603-632 amino acids from the C-terminal region of human CAPN3.

Conjugate:

Unconjugated

Tested Applications:

Flow, IHC-P, WB

Application Note:

For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50

Predicted Molecular Weight:

94 kDa

Advanced Validation

Properties

Purification:

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

CAPN3

Additional Names:

Calpain-3, Calcium-activated neutral proteinase 3, CANP 3, Calpain L3, Calpain p94, Muscle-specific calcium-activated neutral protease 3, New calpain 1, nCL-1, CAPN3, CANP3, CANPL3, NCL1

Protein Accession Number:

P20807

Ncbi Gene Id Number:

825

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A.

Background Reference 1:

Charlton, R., et al. Neuromuscul. Disord. 19(7):449-457(2009)

Background Reference 2:

Moretti, D., et al. Carcinogenesis 30(6):960-967(2009)

Background Reference 3:

Enjuanes, A., et al. Cancer Res. 68(24):10178-10186(2008)

Background Reference 4:

Beckmann, J.S., et al. Neuromuscul. Disord. 18(12):913-921(2008)

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-130

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

Cookie	Duration	Description
cookielawinfo-checbox-analytics	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Analytics".
cookielawinfo-checbox-functional	11 months	The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional".
cookielawinfo-checbox-others	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other.
cookielawinfo-checkbox-necessary	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Necessary".
cookielawinfo-checkbox-performance	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Performance".
viewed_cookie_policy	11 months	The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. It does not store any personal data.

CAPN3 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Homology:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)

HIV-1 p24 Antibody [9D2F6] (biotin)

HIV-1 p24 Antibody [9D2F6]