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Western blot analysis of CAPN6 in rat lung tissue lysate with CAPN6 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of CAPN6 in human lung tissue with CAPN6 antibody at 2.5 μg/mL.
Immunofluorescence of CAPN6 in Human Lung cells with CAPN6 antibody at 20 μg/mL.
Western blot analysis of CAPN6 in rat lung tissue lysate with CAPN6 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

CAPN6 Antibody

CATALOG NUMBER: 4759

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CAPN6 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human CAPN6.
The immunogen is located within the last 50 amino acids of CAPN6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CAPN6 antibody can be used for the detection of StrepII by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1462 - Rat Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Purification:
CAPN6 Antibody is Protein A purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CAPN6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CAPN6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CAPN6
Additional Names:
CAPN6 Antibody: CANPX, CAPNX, CalpM, DJ914P14.1, CALPM, CANPX, Calpain-6, Calpain-like protease X-linked
Protein Accession Number:
NP_055104
Protein Gi Number:
13186316
Ncbi Gene Id Number:
827
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CAPN6 Antibody: Calpains make up a ubiquitously expressed, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. This large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes as their activation can be triggered by calcium influx and oxidative stress. Calpain 6 (CAPN6) is most similar to Calpain 5; the C-terminal region of CAPN6 lacks homology to the calmodulin-like domain of other vertebrate calpains. CAPN6 is thought to be involved in the regulation of microtubule dynamics and cytoskeletal organization. CAPN6 has also been recently identified as an HIV dependency factor (HDF), suggesting that CAPN6 may be an important drug target in HIV treatment.
Background Reference 1:
Croall DE and Ersfeld K. The calpains: modular designs and functional diversity. Genome Biol.2007; 8:216.
Background Reference 2:
Dear N, Matena K, Vingron M, et al. A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution. Genomics1997; 45:175-84.
Background Reference 3:
Tonami K, Kurihara Y, Aburatani J, et al. Calpain 6 is involved in microtubule stabilization and cytoskeletal organization. Mol. Cell. Biol.2007; 27:2548-61.
Background Reference 4:
Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4759

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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