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Western blot analysis of CAPS1 in rat brain tissue lysate with CAPS1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of CAPS1 in human brain with CAPS1 antibody at 5 μg/mL.
Immunofluorescence of CAPS1 in human brain tissue with CAPS1 antibody at 20 μg/mL.
Western blot analysis of CAPS1 in rat brain tissue lysate with CAPS1 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

CAPS1 Antibody

CATALOG NUMBER: 4559

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CAPS1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human CAPS1.
The immunogen is located within the last 50 amino acids of CAPS1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CAPS1 antibody can be used for detection of CAPS1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Numerous isoforms of CAPS1 are known to exist; the lower molecular weight bands seen in the immunoblot image are likely to be these isoforms. This CAPS1 antibody is predicted to be specific to CAPS1 and not recognize CAPS2.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
CAPS1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CAPS1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CAPS1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CADPS
Additional Names:
CAPS1 Antibody: CAPS, CAPS1, CADPS1, CAPS, KIAA1121, Calcium-dependent secretion activator 1, Calcium-dependent activator protein for secretion 1, CAPS-1
Protein Accession Number:
NP_899631
Protein Gi Number:
34452713
Ncbi Gene Id Number:
8618
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CAPS1 Antibody: CAPS1 and its related protein CAPS2 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficienct neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. CAPS1 acts at a stage in exocytosis that follows ATP-dependent priming, which involves the essential synthesis of phosphatidylinositol 4, 5-bisphosphate and is thought to be a specific regulator of large dense-core vesicle fusion.
Background Reference 1:
Walent JH, Porter BW, and Martin TF. A novel 145 kD brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells. Cell1992; 70:765-775.
Background Reference 2:
Juckusch WJ, Speidel D, Sigler A, et al. CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins. Cell2007; 131:796-808.
Background Reference 3:
Rupnick M, Kreft M, Sikdat SK, et al. Rapid regulated dense-core vesicle exocytosis requires the CAPS protein. Proc. Natl. Acad. Sci. USA2000; 97:5627-32.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4559

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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