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Western blot analysis of various lysates, using CAR/CXADR Rabbit mAb at 1:10000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 60s.
Confocal imaging of Hep G2 and K-562(negative sample) cells using CAR/CXADR Rabbit mAb(Red). The cells were counterstained with α-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Western blot analysis of various lysates, using CAR/CXADR Rabbit mAb at 1:10000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 60s.

CAR/CXADR Rabbit Recombinant Antibody (82-307)

CATALOG NUMBER: 82-307

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, WB
Application Note:
WB,1:2000 - 1:10000, IF/ICC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
40kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CXADR
Additional Names:
CAR; HCAR; CAR4/6; CAR/CXADR
Protein Accession Number:
P78310
Ncbi Gene Id Number:
1525
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-307

List Size:
100 uL

List Price:

$477.00

Datasheet
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