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Western blot analysis of Carabin in Daudi cell lysate with Carabin antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of Carabin in human spleen tissue with Carabin antibody at 2.5 μg/mL.
Western blot analysis of Carabin in Daudi cell lysate with Carabin antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Carabin Antibody

CATALOG NUMBER: 4267

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Carabin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Carabin.
The immunogen is located within the last 50 amino acids of Carabin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
Carabin antibody can be used for detection of Carabin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 2:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 3:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
Carabin Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Carabin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Carabin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TBC1D10C
Additional Names:
Carabin Antibody: EPI64C, CARABIN, Carabin, TBC1 domain family member 10C
Protein Accession Number:
NP_940919
Protein Gi Number:
38348348
Ncbi Gene Id Number:
374403
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Carabin Antibody: Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.
Background Reference 1:
Weil R and Israel A. Deciphering the pathway from the TCR to NF-kappaB. Cell Death Differ.2006; 13:826-33.
Background Reference 2:
Im SH and Rao A. Activation and deactivation of gene expression Ca2+/calcineurin-NFAT-mediated signaling. Mol. Cells2004; 18:1-9.
Background Reference 3:
Pan F, Sun L, Kardian DB, et al. Feedback inhibition of calcineurin and ras by a dual inhibitory protein carabin. Nature2007; 445:433-6.
Background Reference 4:
White RA, Pasztor LM, Richardson PM, et al. The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, Bub2, and cdc16 maps to mouse chromosome 5 and human chromosome 4. Cytogenet. Cell Genet.2000; 89:272-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4267

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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