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43-460 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-460 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-460 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

carbonic anhydrase XII Antibody

CATALOG NUMBER: 43-460

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SHLQHVKYKGQE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in Human Kidney lysates (calculated MW of 39.5kDa according to NP_001209.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows membranous staining in some epithelial cells of convoluted tubules. Recommended concentration, 5-10ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_001209.1; NP_996808.1; NP_001280571.1).
Predicted Molecular Weight:
Approx 45kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
carbonic anhydrase XII
Additional Names:
CA12, carbonic anhydrase XII, CAXII, HsT18816, CA-XII, carbonate dehydratase XII, carbonic anhydrase 12, carbonic dehydratase, tumor antigen HOM-RCC-3.1.3
Protein Accession Number:
NP_001209.1,NP_996808.1,NP_001280571.1
Protein Gi Number:
4502515
Ncbi Gene Id Number:
771
Background Reference 1:
Muhammad E et al.Autosomal recessive hyponatremia due to isolated salt wasting in sweat associated with a mutation in the active site of Carbonic Anhydrase 12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-460

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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