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Antibody used in IHC on Human Smooth Muscle.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IHC on Human Smooth Muscle.

CARD12 Antibody

CATALOG NUMBER: 30-999

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CARD12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
CARD12 antibody can be used for detection of CARD12 by ELISA at 1:62500. CARD12 antibody can be used for detection of CARD12 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
116 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CARD12 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NLRC4
Additional Names:
CARD12, CLAN, IPAF, CLAN1, CLANA, CLANB, CLANC, CLAND, CARD12, CLR2.1
Protein Accession Number:
NP_067032
Protein Gi Number:
40788015
Ncbi Gene Id Number:
58484
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1, NOD1, and NOD2. These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.
Background Reference 1:
Clark, H.F., et al., (2003) Genome Res. 13 (10), 2265-2270.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-999

List Size:
100 ul

List Price:

$519.00

Datasheet
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