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Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 μg/ml.
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 μg/mL.
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 μg/mL.
Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

CARD8 Antibody

CATALOG NUMBER: 3187

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8.
The immunogen is located within the last 50 amino acids of CARD8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
CARD8 antibody can be used for detection of CARD8 by Western blot at 1 -2 μg/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1302 – Human Lung Tissue Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Predicted Molecular Weight:
Predicted: 47 kDa
Observed: 47 kDa
Purification:
CARD8 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CARD8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CARD8 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CARD8
Additional Names:
CARD8 Antibody: NDPP, DACAR, DAKAR, NDPP1, TUCAN, CARDINAL, KIAA0955, Caspase recruitment domain-containing protein 8, Apoptotic protein NDPP1
Protein Accession Number:
AAG50014
Protein Gi Number:
12247742
Ncbi Gene Id Number:
22900
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CARD8 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-κB activation.
Background Reference 1:
Scholl FA, McLoghlin P, Ehler E, et al. DRAL is a p53-responsive gene whose four and a half LIM domain protein product induces apoptosis. J. Cell Biol. 2000; 151:495-506.
Background Reference 2:
Stil R, Leonardi A, Formisano L, et al. TUCAN/CARDINAL and DRAL participate in a B activation. FEBS 2002;kcommon pathway for modulation of NF- 521:165-9.
Background Reference 3:
Razmara M, Srinivasula SM, Wang L, et al. CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis. J. Biol. Chem. 2002; 277:13952-8.
Background Reference 4:
Pathan N, Marusawa H, Krajewska M, et al. TUCAN, an antiapoptotic caspase-assoicated recruitment domain family protein overexpressed in cancer. J. Biol. Chem. 2001; 276:32220-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3187

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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