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Western blot analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 μg/ml.
Immunocytochemistry of CARD9 in K562 cells with CARD9 antibody at 10 μg/mL.
Flow Cytometry Validation of CARD9 in Jurkat Cells 1X10^6/mL Jurkat cells were intracellularly stained with 2 μg/mL of CARD9 antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
Western blot analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 μg/ml.
Trial Size

CARD9 Antibody

CATALOG NUMBER: 2515

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (88%), Rat: (82%)
Immunogen:
CARD9 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CARD9.
The immunogen is located within the last 50 amino acids of CARD9.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, ICC, WB
Application Note:
WB: 2.5 μg/mL; ICC: 10 μg/mL; FC: 2 μg/mL.
Antibody validated: Western Blot, Immunocytochemistry and Flow Cytometry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1216 - PC-3 Cell Lysate
Positive Control 2:
Cat. No. 1217 - MDA-MB-361 Cell Lysate
Positive Control 3:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 4:
Cat. No. 17-004 - K-562 Cell Slide
Predicted Molecular Weight:
59 kDa
Purification:
CARD9 Antibody is DEAE purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CARD9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
CARD9 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CARD9
Additional Names:
CARD9 Antibody: CANDF2, hCARD9, Caspase recruitment domain-containing protein 9
Protein Accession Number:
AF311287
Protein Gi Number:
11066983
Ncbi Gene Id Number:
64170
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CARD9 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10. CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-κB. CARD9 is an upstream activator of BCL-10 and NF-κB signaling.
Background Reference 1:
Bertin J, Guo Y, Wang L, Srinivasula SM, Jacobson MD, Poyet JL, Merriam S, Du MQ, Dyer MJ, Robison KE, DiStefano PS, Alnemri ES. CARD9 is a novel caspase recruitment domain-containing protein that interacts with BCL10/CLAP and activates NF-κ B. J Biol Chem. 2000;275(52):41082-6. (RD0202)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2515

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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