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Western blot analysis of CARM1 in Jurkat cell lysate with CARM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of CARM1 in Jurkat cells with CARM1 antibody at 5 μg/mL.
Western blot analysis of CARM1 in Jurkat cell lysate with CARM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

CARM1 Antibody

CATALOG NUMBER: 7219

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal CARM1 antibody was raised against a 19 amino acid peptide near the amino terminus of human CARM1.
The immunogen is located within amino acids 470 - 520 of CARM1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
CARM1 antibody can be used for detection of CARM1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Predicted Molecular Weight:
Predicted: 67 kDa
Purification:
CARM1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CARM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CARM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CARM1
Additional Names:
CARM1 Antibody: PRMT4, PRMT4, Histone-arginine methyltransferase CARM1, Coactivator-associated arginine methyltransferase 1
Protein Accession Number:
NP_954592
Protein Gi Number:
40288288
Ncbi Gene Id Number:
10498
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CARM1 Antibody: Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation. CARM1 functions as a transcriptional co-activator for various nuclear receptors and NF-κB. It has also been shown to methylate histone H3 arginines, inhibiting the binding of corepressors and protecting chromatin from deacetylation, thereby facilitating transcription.
Background Reference 1:
Frankel A, Yadav N, Lee J, et al. The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J. Biol. Chem. 2002; 277:3537-43.
Background Reference 2:
Wolf SS. The protein arginine methyltransferase family: an update about function, new perspectives and the physiological role in humans. Cell Mol. Life Sci. 2009; 66:2109-21.
Background Reference 3:
Lee DY, Teyssier C, Strahl BD, et al. Role of protein methylation in regulation of transcription. Endocr. Rev. 2005; 26:147-70.
Background Reference 4:
Covic M, Hassa PO, Saccani S, et al. Arginine methyltransferase CARM1 is a promoter-specific regulator of NF-kappaB dependent gene expression. EMBO J. 2005; 24:85-96.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7219

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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