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Immunohistochemistry of CARMA3 in human kidney tissue with CARMA3 antibody at 5 μg/ml
Immunohistochemistry of CARMA3 in human kidney tissue with CARMA3 antibody at 5 μg/ml
Trial Size

CARMA3 Antibody

CATALOG NUMBER: 3193

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (81%)
Immunogen:
CARMA3 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CARMA3.
The immunogen is located within the first 50 amino acids of CARMA3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P
Application Note:
CARMA3 antibody can be used for detection of CARMA3 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
CARMA3 antibody is human specific. At least three isoforms of CARMA3 are known to exist; this antibody will only detect isoform 1. CARMA3 antibody is predicted not to cross-react with other CARMA proteins.
Positive Control 1:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
CARMA3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CARMA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CARMA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CARD10
Additional Names:
CARMA3 Antibody: BIMP1, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3, Carma 3
Protein Accession Number:
NP_055365
Protein Gi Number:
51093861
Ncbi Gene Id Number:
29775
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CARMA3 Antibody: CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. As with CARMA1, the CARD domain of CARMA3 has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-κB activation. When expressed in cells, this protein binds to BCL10 and activates NF-κB Recent experiments have shown that CARMA3 is required for EGF-induced NF-κB activation and contributes to tumor growth in vivo, suggesting that CARMA3 may serve as a new therapeutic target for the treatment of EGFR-driven tumors.
Background Reference 1:
Fanning AS and Anderson JM. Protein modules as organizers of membrane structure. Curr. Opin. Cell Biol. 1999; 11:432-9.
Background Reference 2:
Gaide O, Martinon F, Michau O, et al. Carma1, 1 CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappa B activation. FEBS Lett. 2001; 496:121-7.
Background Reference 3:
Wang L, Guo Y, Huang WJ, et al. Card10 is a novel caspase recruitment domain/membrane-associated guanylate kinase family member that interacts with BCL10 and activates NF-kappaB. J. Biol. Chem. 2001; 276:21405-9.
Background Reference 4:
Jiang T, Grabiner B, Zhu Y, et al. CARMA3 is crucial for EGFR-induced activation of NF-kappaB and tumor progression. Cancer Res. 2011; 71:2183-92.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3193

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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