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Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody (15-823) at 1:5000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of HeLa cells transfected with Cas9 (upper left) and untreated HeLa cells (upper right) using CAS9 Rabbit pAb (red, 15-823) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody (15-823) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

CAS9 Antibody

CATALOG NUMBER: 15-823

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Bacteria
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Bacteria
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:5000 - 1:10000
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Predicted Molecular Weight:
Observed: 150kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CAS9
Additional Names:
SPy_1046, SPy1046, 0
Ncbi Gene Id Number:
901176
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-823

List Size:
100 uL

List Price:

$436.00

Datasheet
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