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45-362 (0.3ug/ml) staining of HEK 293 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-362 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
45-362 (0.3ug/ml) staining of HEK 293 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Casein Kinase 1 Antibody

CATALOG NUMBER: 45-362

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in lysates of cell line HEK 293 (calculated MW of 47.3kDa according to NP_001884.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen in the nuclei of U251 cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_001884.2 and NP_620693.1)
Positive Control 1:
Cat. No. 1210 - 293 Whole Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CSNK1D
Additional Names:
casein kinase 1, delta, HGNC:2452, CSNK1D, HCKID, CKIdelta
Protein Accession Number:
NP_001884.2,NP_620693.1
Protein Gi Number:
20149530
Ncbi Gene Id Number:
1453
Background Reference 1:
Xu Y, Padiath QS, Shapiro RE, Jones CR, Wu SC, Saigoh N, Saigoh K, Ptacek LJ, Fu YH. Functional consequences of a CKIdelta mutation causing familial advanced sleep phase syndrome. Nature. 2005 Mar 31;434(7033):640-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-362

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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