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Western blot analysis of CASKIN2 HeLa cell tissue lysate with CASKIN2 antibody at 1 μg/mL.
Immunocytochemistry of CASKIN2 in HeLa cells with CASKIN2 antibody at 2.5 μg/mL.
Immunofluorescence of CASKIN2 in HeLa cells with CASKIN2 antibody at 20 μg/mL.
Western blot analysis of CASKIN2 HeLa cell tissue lysate with CASKIN2 antibody at 1 μg/mL.
Trial Size

CASKIN2 Antibody

CATALOG NUMBER: 6663

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen:
CASKIN2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human CASKIN2.
The immunogen is located within amino acids 380 - 430 of CASKIN2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
CASKIN2 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
CASKIN2 antibody is predicted to not cross-react with other CASKIN family members. Three isoforms of CASKIN2 are known to exist; this antibody will not detect the shortest isoform.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 123, 132 kDa
Observed: 120 kDa
Purification:
CASKIN2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CASKIN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CASKIN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CASKIN2
Additional Names:
CASKIN2 Antibody: ANKS5B, KIAA1139, Caskin-2, CASK-interacting protein 2
Protein Accession Number:
NP_065804
Protein Gi Number:
217416347
Ncbi Gene Id Number:
57513
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CASKIN2 Antibody: Calcium/calmodulin-dependent serine protein kinase (CASK) is a conserved multi-domain scaffolding protein involved in brain development, synapse formation, and establishment of cell polarity. CASKINs (CASK interacting protein ) interact with CASK and is thought to play a role in CASK function, specifically by coupling CASK to distinct downstream effectors. CASKIN2 is a multidomain protein containing six N-terminal ankyrin repeats, one SH3 domain, and two sterile alpha motif domains followed by a long proline-rich sequence and a short conserved C-terminal domain. It is expressed ubiquitously with highest levels present in fetal and adult liver tissue.
Background Reference 1:
Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13
Background Reference 2:
Weng YL, Liu N, DiAntonio A, et al. The cytoplasmic adaptor protein Caskin mediates Lar signal transduction during Drosophila motor axon guidance. Neurosci. 2011; 31:4421-33.
Background Reference 3:
Tabuchi K, Biederer T, Butz S, et al. CASK participates in alternative tripartite complexes in which Mint 1 competes for binding with CASKIN2, a novel CASK-binding protein. J. Neurosci. 2002; 22:4264-73.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6663

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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