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Western blot analysis of extracts of 293T cells, using CASP2 antibody (13-794).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse kidney using CASP2 antibody (13-794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using CASP2 antibody (13-794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using CASP2 antibody (13-794) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CASP2 antibody (13-794).
Immunofluorescence analysis of A549 cells using CASP2 antibody (13-794).
Western blot analysis of extracts of 293T cells, using CASP2 antibody (13-794).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

CASP2 Antibody [C1F6]

CATALOG NUMBER: 13-794

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human CASP2
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
293T
Predicted Molecular Weight:
Observed: 51kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CASP2
Additional Names:
Caspase-2 Antibody: ICH1, NEDD2, CASP-2, NEDD-2, PPP1R57, ICH1, Caspase-2, Neural precursor cell expressed developmentally down-regulated protein 2
Ncbi Gene Id Number:
835
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Caspases mediate cellular apoptosis through the proteolytic cleavage of specific protein substrates. The encoded protein may function in stress-induced cell death pathways, cell cycle maintenance, and the suppression of tumorigenesis. Increased expression of this gene may play a role in neurodegenerative disorders including Alzheimer's disease, Huntington's disease and temporal lobe epilepsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-794

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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