Western blot analysis of lysates from EL4 cells using Caspase-1 Rat mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rat IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.

Western blot analysis of lysates from IBMDM cells using Caspase-1 Rat mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. IBMDM cells treated with LPS (100 ng/ml) for 4 hours and Nigericin (20uM) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rat IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90 s.

Western blot analysis of lysates from EL4 cells using Caspase-1 Rat mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rat IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 45s.

Caspase-1 Rat Recombinant Antibody (81-873)

CATALOG NUMBER: 81-873

Clonality:

Monoclonal

Tested Applications:

ELISA, WB

Host Species:

Rat

Species Reactivity:

Mouse

Conjugate:

Unconjugated

Specifications

Host Species:

Rat

Species Reactivity:

Mouse

Immunogen:

Recombinant protein (or fragment).This information is considered to be commercially sensitive.

Conjugate:

Unconjugated

Tested Applications:

ELISA, WB

Application Note:

WB,1:1000 - 1:3000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Predicted Molecular Weight:

46kDa

Advanced Validation

Properties

purification:

Affinity purification

Clonality:

Monoclonal

Isotype:

IgG

physical-state:

Liquid

Buffer:

Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Concentration:

Batch dependent

Storage Conditions:

Store at -20℃. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

Casp1

Additional Names:

ICE; Il1bc

Protein Accession Number:

P29452

Ncbi Gene Id Number:

12362

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

Enables cysteine-type endopeptidase activity. Involved in several processes, including positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-1 beta production; and protein autoprocessing. Acts upstream of or within several processes, including membrane hyperpolarization; mitochondrial depolarization; and positive regulation of interleukin-1 alpha production. Located in extracellular region. Part of IPAF inflammasome complex. Is expressed in knee joint and metanephros. Orthologous to human CASP1 (caspase 1).

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

CATALOG NUMBER:

81-873

List Size:

100 uL

List Price:

$477.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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Bulk Pricing Available

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Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

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Caspase-1 Rat Recombinant Antibody (81-873)

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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