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Caspase-12 Antibody [12G6]

CATALOG NUMBER: 36-184

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, WB
Host Species:
Rat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rat
Species Reactivity:
Mouse
Immunogen:
Recombinant mouse caspase-12 (p20 fragment) and synthetic peptide corresponding to aa 183-205 of mouse caspase-12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, WB
Application Note:
ELISA: (direct and indirect: 1:2,000-1:5,000). Immunoprecipitation: (1:500-1:5,000). Western Blot: (1:500-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Specificity:
Recognizes an epitope within the p20 fragment of mouse caspase-12. Detects a band of ~51kDa by Western blot. Does not cross-react with mouse caspase-1 or -11.
Purification:
>95% (SDS-PAGE)
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
Liquid. In PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
Stable for at least 1 year after receipt when stored at -20°C.
Ncbi Official Symbol:
Casp12
Additional Names:
CASP-12
Protein Accession Number:
2094806
Protein Gi Number:
CAA73532
Ncbi Gene Id Number:
12364
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

36-184

List Size:
100 ug

List Price:

$463.00

Datasheet
Shipping Info

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