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Western blot analysis of caspase-12 in (A) human and (B) mouse spleen tissue lysates with caspase-12 antibody at 1 μg/mL.
Immunohistochemistry of caspase-12 in human heart tissue with caspase-12 antibody at 10 μg/mL.
Western blot analysis of caspase-12 in (A) human and (B) mouse spleen tissue lysates with caspase-12 antibody at 1 μg/mL.
Trial Size

Caspase-12 Antibody

CATALOG NUMBER: 2325

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Caspase-12 antibody was raised against a 16 amino acid peptide near the amino terminus of murine Caspase-12.
The immunogen is located within the first 50 amino acids of Caspase-12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
Caspase-12 antibody can be used for detection of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human and mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Positive Control 2:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 3:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 4:
Cat. No. 10-501 - Human Heart Tissue Slide
Predicted Molecular Weight:
Predicted: 46 kDa
Observed: 51 kDa
Purification:
Caspase-12 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Caspase-12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Casp12
Additional Names:
Caspase-12 Antibody: Caspase-12, CASP-12
Protein Accession Number:
CAA73532
Protein Gi Number:
2094806
Ncbi Gene Id Number:
12364
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Caspase-12 Antibody: Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
Background Reference 1:
Nakagawa T, Zhu H, Morishima N, et al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
Background Reference 2:
Mehmet H. Caspases find a new place to hide. Nature 2000; 403:29-30
Background Reference 3:
Van de Craen M, Vandenabeele P, Declercq W, et al. Characterization of seven murine caspase family members. FEBS Lett 1997; 403:61-9

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2325

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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