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<strong> Figure 1 WB Validation in Rat Tissues </strong><br>Loading: 15 μg of lysate Antibodies: Caspase-12, 3195 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 WB Validation in Human and Mouse Heart </strong><br>Loading: 15 μg of lysate Antibodies: Caspase-12, 3195, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Human heartLane B: Mouse heart
<strong> Figure 3 WB Validation in Human Tissues </strong><br>Loading: 15 μg of lysate Antibodies: Caspase-12, 3195 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of Caspase-12 in Mouse Brain</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Caspase-12 antibody (3195) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunohistochemistry Validation of Caspase-12 in Rat Brain</strong><br> Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Caspase-12 antibody (3195) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunohistochemistry Validation of Caspase-12 in Human Colon</strong><br> Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Caspase-12 antibody (3195) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunofluorescence Validation of Caspase-12 in Mouse Heart</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart labeling caspase-12 with 3195 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
Figure 1 WB Validation in Rat Tissues
Loading: 15 μg of lysate Antibodies: Caspase-12, 3195 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Cell Treatment
Trial Size

Caspase-12 Antibody (Large)

CATALOG NUMBER: 3195

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-Caspase-12 antibody (3195) was raised against a peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12.

The immunogen is located within amino acids 170 - 220 of Caspase-12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Application Note:
WB: 0.5-2 μg/mL; IHC-P: 0.5-2 μg/mL; IHC-P/IF: 10-20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
Caspase-12 (large) antibody is predicted to have no cross reactivity to other members in the caspase family.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 3:
Cat. No. 1461 - Rat Heart Tissue Lysate
Predicted Molecular Weight:
Predicted: 48 kD

Observed: 46 kD
Isoforms:
Mouse Caspase-12 has one isoform (419aa, 48kD). Rat Caspase-12 has one isoform (420aa, 48kD) and human Caspase-12 also has two isoforms (39kD and 19kD). 3195 can detect human, mouse and rat.
Purification:
Caspase-12 (Large) Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Large)
Concentration:
1 mg/mL
Storage Conditions:
Caspase-12 (Large) antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Casp12
Additional Names:
Caspase-12 (Large) Antibody: Caspase-12, CASP-12
Protein Accession Number:
CAA73532
Protein Gi Number:
2094806
Ncbi Gene Id Number:
12364
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Caspase-12 Antibody: (Large)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
Background Reference 1:
Nakagawa et al. Nature. 2000; 403:98-103.
Background Reference 2:
Mehmet H. Nature 2000; 403:29-30
Background Reference 3:
Van de Craen et al. FEBS Lett. 1997; 403:61-9

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3195

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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