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Western blot analysis of caspase-12 in mouse (lane A) and rat (lane B) liver lysate with caspase-12 antibody (small) at 1 μg/mL.
Immunohistochemical staining of human liver tissue using caspase-12 antibody (small) at 10 μg/mL.
Western blot analysis of caspase-12 in mouse (lane A) and rat (lane B) liver lysate with caspase-12 antibody (small) at 1 μg/mL.
Trial Size

Caspase-12 Antibody (Small)

CATALOG NUMBER: 3197

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
Caspase-12 antibody was raised against a synthetic peptide corresponding to 15 amino acids within the small cleavage product of murine caspase-12.
The immunogen is located within the last 50 amino acids of Caspase-12 .
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
Caspase-12 (small) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in mouse and rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Positive Control 2:
Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 3:
Cat. No. 10-201 - Human Liver Tissue Slide
Predicted Molecular Weight:
Predicted: 46 kDa (uncleaved)
Observed: 38 kDa
Purification:
Caspase-12 Antibody (Small) is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Small)
Concentration:
1 mg/mL
Storage Conditions:
Caspase-12 antibody (Small) can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Casp12
Additional Names:
Caspase-12 Antibody (Small): Caspase-12, CASP-12
Protein Accession Number:
CAA73532
Protein Gi Number:
2094806
Ncbi Gene Id Number:
12364
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Caspase-12 Antibody: (Small)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
Background Reference 1:
Nakagawa T, Zhu Human, Mouseorishima N, el al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
Background Reference 2:
Mehmet H. Caspases find a new place to hide. Nature 2000; 403:29-30
Background Reference 3:
Van de Craen M, Vandenabeele P, Declercq W,et al. Characterization of seven murine caspase family members. FEBS Lett 1997; 403:61-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3197

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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