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Western blot analysis of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 μg/mL.
Immunohistochemistry of caspase-14 in human liver tissue with caspase-14 antibody at 2.5 μg/mL.
Western blot analysis of caspase-14 in Jurkat cell lysate in the (A) absence or (B) presence of blocking peptide with caspase-14 antibody at 1 μg/mL.
Trial Size

Caspase-14 Antibody

CATALOG NUMBER: 2509

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Caspase-14 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human caspase-14.
The immunogen is located within the last 50 amino acids of Caspase-14.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
Caspase-14 antibody can be used for the detection of caspase-14 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 3:
Cat. No. 10-201 - Human Liver Tissue Slide
Predicted Molecular Weight:
Predicted: 28 kDa
Observed: 27 kDa
Purification:
Caspase-14 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Caspase-14 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Caspase-14 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Casp14
Additional Names:
Caspase-14 Antibody: MICE, mini-ICE, Caspase-14, Mini-ICE, CASP-14
Protein Accession Number:
ACA63845
Protein Gi Number:
169666460
Ncbi Gene Id Number:
23581
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Caspase-14 Antibody: Caspases are a family of cysteine proteases that can be divided into apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1b and IL-18. Caspase-14 is highly expressed in embryonic but not adult tissues. It is processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in the keratinocyte terminal differentiation, which is important for the formation of the skin barrier.
Background Reference 1:
Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
Background Reference 2:
Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
Background Reference 3:
Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme. Science 1995; 267:2000-3.
Background Reference 4:
Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2509

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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