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Western blot analysis of Caspase-2 in (A) human thymus tissue and (B) human kidney lysate with Caspase-2 antibody at 1 μg/mL.
Immunocytochemistry of caspase-2 in A-20 cells with caspase-2 antibody at 2 μg/mL.
Western blot analysis of Caspase-2 in (A) human thymus tissue and (B) human kidney lysate with Caspase-2 antibody at 1 μg/mL.
Trial Size

Caspase-2 Antibody

CATALOG NUMBER: 3447

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%), Chicken: (75%)
Immunogen:
Caspase-2 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-2.
The immunogen is located within the first 50 amino acids of Caspase-2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
Caspase-2 antibody can be used for the detection of caspase-2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
Depending on cell lines or tissues used, other cleavage products may be observed.
Positive Control 1:
Cat. No. 1314 - Human Thymus Tissue Lysate
Positive Control 2:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 3:
Cat. No. 1288 - A20 Cell Lysate
Predicted Molecular Weight:
Predicted: 51 kDa
Observed: 50 kDa
Purification:
Caspase-2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Caspase-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Caspase-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CASP2
Additional Names:
Caspase-2 Antibody: ICH1, NEDD2, CASP-2, NEDD-2, PPP1R57, ICH1, Caspase-2, Neural precursor cell expressed developmentally down-regulated protein 2
Protein Accession Number:
P42575
Protein Gi Number:
83300977
Ncbi Gene Id Number:
835
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Caspase-2 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Although phylogenetically similar to this subfamily, Caspase-2 is thought to be involved in stress-induced apoptosis. Caspase-2 has two major isoforms; overexpression on the long form results in apoptosis while that of the short form suppresses cell death.
Background Reference 1:
Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
Background Reference 2:
Zhivotovsky B and Orrenius S. Caspase-2 funtion in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
Background Reference 3:
Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
Background Reference 4:
Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3447

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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