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Western blot analysis of Caspase-5 in Ramos cells with Caspase-5 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Western blot analysis of Caspase-5 in (A) human spleen tissue and (B) human thymus lysate with Caspase-5 antibody at 1 μg/mL.
Western blot analysis of Caspase-5 in Ramos cells with Caspase-5 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Trial Size

Caspase-5 Antibody

CATALOG NUMBER: 3455

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Caspase-5 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-5.
The immunogen is located within amino acids 40 - 90 of Caspase-5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Casp-5 antibody can be used for the detection of Caspase-5 by Western blot at 0.5 to 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
Depending on cell lines or tissues used, other cleavage products may be observed.
Positive Control 1:
Cat. No. 1225 - Ramos Cell Lysate
Positive Control 2:
Cat. No. 1314 - Human Thymus Tissue Lysate
Positive Control 3:
Cat. No. 1306 - Human Spleen Tissue Lysate
Predicted Molecular Weight:
Predicted: 48 kDa
Observed: 48 kDa
Purification:
Caspase-5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Caspase-5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Caspase-5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CASP5
Additional Names:
Caspase-5 Antibody: ICH-3, ICEREL-III, ICE(rel)III, ICH3, Caspase-5, Protease ICH-3, CASP-5
Protein Accession Number:
P51878
Protein Gi Number:
212276423
Ncbi Gene Id Number:
838
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Caspase-5 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.
Background Reference 1:
Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
Background Reference 2:
Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
Background Reference 3:
Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
Background Reference 4:
Martinon F, Burns K, and Tschopp J. The inflammasome: a molecular platform triggering activation of the inflammatory caspases and processing of proIL-1beta. Mol. Cell. 2002; 10:417-26.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3455

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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