Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: Caspase 9, 2071 (1 μg/mL), Caspase 9, 2073 (1 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: Caspase 9, 2071 (1 μg/mL)), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Heart</strong><br>Loading: 15 μg of lysates per lane.Antibodies: Caspase 9, 2071, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 μg/mLLane 2: 2 μg/mLLane 3: 4 μg/mL
<strong>Figure 4 Western Blot Validation in HeLa Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: Caspase 9, 2071 (Lane 1: 0.5 μg/mL; Lane 2: 1 μg/mL and Lane 3: 2 μg/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Western Blot Validation in Mouse Spleen Tissue</strong><br>Loading: 15 μg of lysates per lane.Antibodies: Caspase 9, 2071 (Lane 1: 0.5 μg/mL; Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.5 μg/mLLane 2: 1 μg/mL and Lane 3: 2 μg/mL
<strong>Figure 6 Western Blot Validation in Rat Spleen Tissue</strong><br>Loading: 15 μg of lysates per lane.Antibodies: Caspase 9, 2071 (Lane 1: 0.5 μg/mL; Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: Caspase 9, 2071 (1 μg/mL), Caspase 9, 2073 (1 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Green Product
IAV
Trial Size

Caspase-9 Antibody

CATALOG NUMBER: 2071

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti- Caspase 9 antibody (2071) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human Caspase 9.
The immunogen is located within amino acids 30-80 of Caspase 9.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.5-2 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested.
Specificity:
It has no cross response to other members in caspase family.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 3:
Cat. No. 1466 - Rat Spleen Lysate
Predicted Molecular Weight:
Predicted: 46kD
Observed: 46kD
Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar Caspase 9 expression profile in human cell lines detected by two independent anti-Caspase 9 antibodies that recognize different epitopes, 2071 against N-terminus domain and 2073 against central domain.  Caspase 9 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

Isoforms:
Human Caspase 9 has 4 isoforms, including isoform 1 (416aa, 46kD), isoform 2 (266aa, 30kD), isoform 3 (154aa, 17kD), and isoform 4 (333aa, 37kD). Mouse Caspase 9 has one isoform (454aa, 50kD) and rat Caspase 9 also has one isoform (454aa, 50kD). 2071 can detect human, mouse and rat.
Purification:
Caspase-9 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Caspase-9 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
Caspase-9 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CASP9
Additional Names:
Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9
Protein Accession Number:
P55211
Protein Gi Number:
28558771
Ncbi Gene Id Number:
842
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
Background Reference 1:
Duan et al. J Biol Chem 1996;271:16720-4.
Background Reference 2:
Srinivasula et al. J Biol Chem 1996;271:27099-106.
Background Reference 3:
Li et al. Cell 1997;91:479-89.
Background Reference 4:
Pan et al. J Biol Chem 1998;273:5841-5 (RD1299).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2071

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369