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Western blot analysis of CASR in EL4 cell lysate with CASR antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of CASR in human kidney tissue with CASR antibody at 5 μg/mL.
Immunofluorescence of CASR in human kidney tissue with CASR antibody at 20 μg/mL.
Western blot analysis of CASR in EL4 cell lysate with CASR antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

CASR Antibody

CATALOG NUMBER: 7785

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
CASR antibody was raised against a 15 amino acid peptide near the amino terminus of human CASR.
The immunogen is located within the first 50 amino acids of CASR.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CASR antibody can be used for detection of CASR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
CASR antibody is human, mouse and rat reactive. At least two isoforms of CASR are known to exist.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 101, 120 kDa
Observed: 115, 125 kDa
Purification:
CASR antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CASR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CASR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CASR
Additional Names:
CASR Antibody: CAR, FHH, FIH, HHC, EIG8, HHC1, NSHPT, PCAR1, GPRC2A, HYPOC1, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, CaSR
Protein Accession Number:
NP_001171536
Protein Gi Number:
296010811
Ncbi Gene Id Number:
846
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The calcium-sensing receptor protein (CASR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (1). It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis (1). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (1,2). Recent evidence suggests that activated CASR contributes to the cytokine secretion through the partial MAPK and NF-kappaB pathways in T cells (3).
Background Reference 1:
Pollak MR, Brown EM, Chou YH, et al. Mutations in the human Ca(2+)-sensing receptor gene cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism. Cell 1993; 75:1297-303.
Background Reference 2:
Jakobsen SF, Rolighed L, Nissen PH, et al. Muscle function and quality of life are not impaired in familial hypocalciuric hypercalcemia: a cross-sectional study on physiological effects of inactivating variants in the calcium-sensing receptor gene (CASR). Eur. J. Endocrinol. 2013; 169:349-57.
Background Reference 3:
Li T, Sun M, Yin X, et al. Expression of the calcium sensing receptor in human peripheral blood T lymphocyte and its contribution to cytokine secretion through MAPKs or NF-kB pathways. Mol. Immunol. 2013; 53:414-20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7785

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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