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Human Cathepsin B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human Cathepsin B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Cathepsin B Recombinant Protein

CATALOG NUMBER: 96-223

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Arg 18 - Ile 339
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
36.7 kDa (pro-form) and 29 kDa (mature-form)
Biological Activity:
Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100μl reaction mixture containing 25 mM MES, pH 5. 0, 0. 01 μg rhCathepsin B, 10 μM reaction substrate. The specific activity is >2800 pmol/min/μg.
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
50 mM Tris, 150 mM NaCl, pH8.0
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CTSB
Additional Names:
CTSB, CPSB, APPS
Protein Accession Number:
NP_001899
Ncbi Gene Id Number:
1508
Background:
Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; Preferentially cleaves -Arg-Arg-, -Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
Background Reference 1:
Basrur V., et al., 2003, J. Proteome Res. 2:69-79.
Background Reference 2:
Chi A., et al., 2006, J. Proteome Res. 5:3135-3144.
Background Reference 3:
Royer-Zemmour B., et al., 2008, Hum. Mol. Genet. 17:3617-3630.
Background Reference 4:
Cao L., et al., 1994, Gene 139 (2): 163–9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-223

List Size:
0.05 mg

List Price:

$540.00

Datasheet
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