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Western blot analysis of CAZIP in A20 cell lysate with CAZIP antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of CAZIP in mouse heart tissue with CAZIP antibody at 5 μg/mL.
Immunofluorescence of CAZIP in mouse heart tissue with CAZIP antibody at 20 μg/mL.
Western blot analysis of CAZIP in A20 cell lysate with CAZIP antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

CAZIP Antibody

CATALOG NUMBER: 5639

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
CAZIP antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human CAZIP.
The immunogen is located within amino acids 1300 - 1350 of CAZIP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CAZIP antibody can be used for detection of CAZIP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Purification:
CAZIP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CAZIP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CAZIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MTUS2
Additional Names:
CAZIP Antibody: ICIS, CAZIP, TIP150, KIAA0774, Microtubule-associated tumor suppressor candidate 2, Cardiac zipper protein, Tracking protein of 150 kDa
Protein Accession Number:
NP_001028774
Protein Gi Number:
140161498
Ncbi Gene Id Number:
23281
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CAZIP Antibody: CAZIP, also known as TIP150, contains a leucine-zipper domain at the C-terminus and has at least three isoforms. CAZIP is expressed in heart during early cardiac development and in parts of the nervous system in later embryonic development. It is thought to bind microtubules and facilitate the end-binding protein 1 (EB1)-dependent loading of MCAK onto microtubules plus ends and orchestrates the dynamics at the plus end of microtubules. CAZIP may regulate the dynamics of microtubules at their growing distal tip.
Background Reference 1:
Du Puy L, Beqqali A, Monshouwer-Kloots J, et al. CAZIP, a novel protein expressed in the developing heart and nervous system. Dev. Dyn.2009; 238:2903-11.
Background Reference 2:
Jiang K, Wang J, Liu J, et al. TIP150 interacts with and targets MCAK at the microtubule plus ends. EMBO Rep.2009; 10:857-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5639

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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