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Immunohistochemical analysis of paraffin-embedded R. brain section using CB2 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. brain section using CB2 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Jurkat cells using CB2 Antibody compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis in A431 cell line lysates (35ug/lane).
CB2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of paraffin-embedded R. brain section using CB2 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

CB2 Antibody

CATALOG NUMBER: 55-328

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-356 amino acids from the C-terminal region of human CB2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CNR2
Additional Names:
Cannabinoid receptor 2, CB-2, CB2, hCB2, CX5, CNR2, CB2A, CB2B
Protein Accession Number:
P34972
Protein Gi Number:
461697
Ncbi Gene Id Number:
1269
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The cannabinoid delta-9-tetrahydrocannabinol is theprincipal psychoactive ingredient of marijuana. The proteinsencoded by this gene and the cannabinoid receptor 1 (brain) (CNR1)gene have the characteristics of a guanine nucleotide-bindingprotein (G-protein)-coupled receptor for cannabinoids. They inhibitadenylate cyclase activity in a dose-dependent, stereoselective,and pertussis toxin-sensitive manner. These proteins have beenfound to be involved in the cannabinoid-induced CNS effects(including alterations in mood and cognition) experienced by usersof marijuana. The cannabinoid receptors are members of family 1 ofthe G-protein-coupled receptors.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Larrinaga, G., et al. Histol. Histopathol. 25(9):1133-1138(2010)
Background Reference 3:
Pinheiro, A.P., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Background Reference 4:
Taylor, A.H., et al. Histochem. Cell Biol. 133(5):557-565(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-328

List Size:
400 ul

List Price:

$519.00

Datasheet
Shipping Info

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