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42-843 (0.5ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-843 (3.75ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-843 (0.5ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CBARA1 Antibody

CATALOG NUMBER: 42-843

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-EYENRIRAYSTPDK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in lysates of cell line K562 (calculated MW of 54.6kDa according to NP_006068.2). Recommended concentration: 0.5-2ug/ml.An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Lung. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize reported isoforms 1 and 2 (NP_006068.2; NP_001182447.1).
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CBARA1
Additional Names:
CBARA1, calcium binding atopy-related autoantigen 1, CALC, DKFZp564C246, EFHA3, FLJ12684, OTTHUMP00000019787, ara CALC, atopy related autoantigen, atopy-related autoantigen CALC, calcium-binding atopy-related autoantigen 1
Protein Accession Number:
NP_006068.2,NP_001182447.1
Protein Gi Number:
116256483
Ncbi Gene Id Number:
10367

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-843

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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