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43-373 (0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-373 (0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CBFB Antibody

CATALOG NUMBER: 43-373

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-EARRQQDPSPGSN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 22kDa band observed in lysates of cell line Daudi (calculated MW of 22.0kDa according to NP_074036.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize reported isoform 1 (NP_074036.1) only, however it is expected to recognize reported mouse isoforms 1 (NP_071704.3), 2 (NP_001154928.1) and 3 (NP_001154929.1).
Predicted Molecular Weight:
Approx 22kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CBFB
Additional Names:
CBFB, core-binding factor, beta subunit, PEBP2B, CBF-beta, PEA2-beta, PEBP2-beta, SL3-3 enhancer factor 1 beta subunit, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, core-binding factor subunit beta, polyomavirus enhancer binding protein 2, beta subunit, polyomavirus enhancer-binding protein 2 beta subunit
Protein Accession Number:
NP_074036.1,NP_001746.1
Protein Gi Number:
13124873
Ncbi Gene Id Number:
865
Background Reference 1:
Hultquist JF, Binka M, LaRue RS, Simon V, Harris RS.Vif proteins of human and simian immunodeficiency viruses require cellular CBFβ to degrade APOBEC3 restriction factors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-373

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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