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Western blot analysis of cbl in Daudi cell lysate with cbl antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunofluorescence of cbl in human lymph node tissue with cbl antibody at 20 μg/mL.
Western blot analysis of cbl in Daudi cell lysate with cbl antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Trial Size

Cbl Antibody

CATALOG NUMBER: 3967

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Cbl antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human cbl.
The immunogen is located within amino acids 810 - 860 of Cbl.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Cbl antibody can be used for detection of cbl by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 2:
Cat. No. 1369 - Human Lymph node Tissue Lysate
Positive Control 3:
Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
Purification:
Cbl Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Cbl Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Cbl antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CBL
Additional Names:
Cbl Antibody: CBL2, NSLL, C-CBL, RNF55, FRA11B, CBL2, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene
Protein Accession Number:
P22681
Protein Gi Number:
115855
Ncbi Gene Id Number:
867
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cbl Antibody: The mammalian cbl family of ubiquitin ligases consists of three homologs known as cbl (also known as c-Cbl), Cbl-B, and Cbl-3 which share highly conserved a tyrosine-kinase-binding domain, linker and RING finger domain in their amino-terminal halves. Similar to other E3 ubiquitin ligases, Cbl catalyzes the transfer of ubiquitin from an E2 or Ubc (ubiquitin-conjugating) enzyme to the e-amino group of a lysine residue of the substrate protein. Cbl acts to negatively regulate many types of cell-surface receptors, including the Syk protein tyrosine kinase family. Cbl is thought to be involved in T- and B-cell signaling, in addition to thymus development. Of the three known homologs in the cbl family, cbl antibody reacts specifically with cbl. Multiple isoforms of cbl have been reported.
Background Reference 1:
Thien CBF and Langdon WY. C-Cbl and Cbl-b ubiquitin ligases: substrate diversity and the negative regulation of signaling responses. Biochem. J. 2005; 391:153-66
Background Reference 2:
Weissman AM. Themes and variations on ubiquitylation. Nat. Rev. Mol. Cell Biol. 2001; 2:169-78.
Background Reference 3:
Swaminathan G and Tsygankov AY. The Cbl family of proteins: ring leaders in regulation of cell signaling. J. Cell. Physiol. 2006; 209:21-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3967

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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