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45-369 (0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-369 (0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CBR3 Antibody

CATALOG NUMBER: 45-369

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QGQLVHDKVVQNW
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in lysates of cell line HEK293 (calculated MW of 30.9kDa according to NP_001227.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bains et al, J Pharmacol Exp Ther. 2010 Mar;332(3):755-63.; PMID: 20007405). Recommended concentration: 1-3ug/ml. An anonymous customer found positive results in WB on Human recombinant protein.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CBR3
Additional Names:
CBR3, carbonyl reductase 3, carbonyl reductase (NADPH) 3, SDR21C2, hCBR3, NADPH-dependent carbonyl reductase 3, short chain dehydrogenase/reductase family 21C, member 2
Protein Accession Number:
NP_001227.1
Protein Gi Number:
4502601
Ncbi Gene Id Number:
874
Background Reference 1:
Watanabe K, Sugawara C, Ono A, Fukuzumi Y, Itakura S, Yamazaki M, Tashiro H, Osoegawa K, Soeda E, Nomura T. Mapping of a novel human carbonyl reductase, CBR3, and ribosomal pseudogenes to human chromosome 21q22.2. Genomics. 1998 Aug 15;52(1):95-100.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-369

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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